黄秋葵(Abelmoschus esculentus L.)再生体系建立和愈伤组织花青素诱导研究

硕士博士毕业论文站内搜索    
分类:教育论文网→农业科学论文→园艺论文其他蔬菜论文
黄秋葵(Abelmoschus esculentus L.)再生体系建立和愈伤组织花青素诱导研究
论文目录
 
List of Abbreviations第11-13页
Chinese abstract第13-16页
Abstract第16-19页
Chapter 1: General Introduction第19-35页
  1.1 Background of okra crop第19-20页
  1.2 Plant tissue culture第20-21页
  1.3 Okra tissue culture第21页
  1.4 Phenolic compounds secretion from explant and their lethal effects on plant tissueculture第21-22页
  1.5 Oxidative browning in plant tissue culture第22-24页
  1.6 Factors affecting phenolic secretion第24页
  1.7 Strategies to overcome phenolic secretion and culture browning第24-25页
  1.8 Mechanism of browning control by additives in culture medium第25-26页
  1.9 Limitations of anti-browning additives in plant tissue culture第26-28页
  1.10 Plant cell and tissue culture an attractive alternative for secondary metabolitesproduction第28-32页
    1.10.1 In vitro anthocyanin production第28-29页
    1.10.2 Factors affecting in vitro anthocyanin production第29页
    1.10.3 Nitrogen deficiency as a way to modulate anthocyanin accumulation in invitro cultures第29-32页
    1.10.4 Light intensity effects anthocyanin accumulation in plant tissues第32页
  1.11 Project aims and objectives第32-35页
Chapter 2: In vitro regeneration of Abelmoschus esculentus L. cv. Wufu: Influence ofanti-browning additives on phenolic secretion and callus formation frequency inexplants第35-57页
  2.1 Introduction第37-38页
  2.2 Materials and methods第38-42页
    2.2.1 Seed material第38-39页
    2.2.2 Media fortification with anti-browning additives第39页
    2.2.3 Callus induction第39页
    2.2.4 Callus growth and development第39-41页
    2.2.5 Shoot induction and elongation第41页
    2.2.6 Root formation and acclimatization of plantlets第41页
    2.2.7 Statistical analysis第41-42页
  2.3 Results第42-52页
    2.3.1 Influence of anti-browning additives on phenolic secretion in callus culture第42-45页
    2.3.2 Influence of plant growth regulators on callus induction第45页
    2.3.3 Interaction effect of anti-browning additives and PGRs on callus induction第45-49页
    2.3.4 Callus growth and development第49页
    2.3.5 Shoot regeneration第49页
    2.3.6 Rooting and acclimatization of plantlets第49-52页
  2.4 Discussion第52-55页
  2.5 Conclusion第55-57页
Chapter 3: Ascorbic acid controls lethal browning and Pluronic F-68 promotes high-frequency multiple shoot regeneration from cotyldonary node explant of okra(Abelmoschusesculentus.L)第57-75页
  3.1 Introduction第59-61页
  3.2 Materials and methods第61-64页
    3.2.1 Preparation of ascorbic acid, silver nitrate, and Pluronic F-68 stock solutions第61页
    3.2.2 Surface sterilization and seed germination第61-62页
    3.2.3 Fortification of anti-browning additives in culture medium第62页
    3.2.4 Culturing of explants on anti-browning additives supplemented medium第62页
    3.2.5 Multiple shoots induction and proliferation第62-63页
    3.2.6 Shoot elongation第63页
    3.2.7 In vitro rooting and acclimatization of plantlets第63页
    3.2.8 Hardening and field transfer第63页
    3.2.9 Statistical analysis第63-64页
  3.3 Results第64-71页
    3.3.1 Culture establishment第64-65页
    3.3.2 Effects of anti-browning additives on phenolic secretion and culture vigor第65-66页
    3.3.3 Effects of plant growth regulators and regeneration enhancers on shoot initiation andmultiplication第66页
    3.3.4 Effects of plant growth regulators and regeneration enhancers on phenotypes ofregenerated shoots第66-68页
    3.3.5 Shoot elongation第68-69页
    3.3.6 In vitro rooting and acclimatization第69-71页
  3.4 Discussion第71-74页
  3.5 Conclusion第74-75页
Chapter 4: Accumulation of anthocyanin in callus cultures of red-pod okra[Abelmoschus esculentus (L.) Hongjiao] in response to light and nitrogen levels第75-95页
  4.1 Introduction第77-79页
  4.2 Materials and methods第79-83页
    4.2.1 Surface sterilization and seed germination第79页
    4.2.2 Explant preparation and callus induction第79页
    4.2.3 Light irradiation and media composition for anthocyanin induction第79-81页
    4.2.4 Determination of callus biomass第81页
    4.2.5 Determination of total anthocyanin (TA) content第81页
    4.2.6 Determination of total phenolic (TP) content第81-82页
    4.2.7 Determination of total antioxidant activity (TAA)第82页
    4.2.8 Total flavonoid (TF) content第82页
    4.2.9 Microscopic analysis第82页
    4.2.10 HPLC analysis第82-83页
    4.2.11 Statistical analysis第83页
  4.3 Results and discussion第83-94页
    4.3.1 Callus culture第83-84页
    4.3.2 Effects of nitrogen level and light intensity on callus biomass accumulation第84-85页
    4.3.3 Effects of different nitrogen concentrations and light irradiation on anthocyanininduction第85-88页
    4.3.4 Callus biomass accumulation and its correlation with anthocyanin accumulation第88-89页
    4.3.5 Phenolic content,flavonid accmulation,and antioxidant activity in callus culturesunder different culture conditions第89-92页
    4.3.6 Pigment composition in callus cultures第92-94页
  4.4 Conclusion第94-95页
Chapter 5: General discussion and conclusions第95-99页
References第99-117页
Appendices第117-159页
Publications第159-161页
Acknowledgements第161页

本篇论文共161页,点击这进入下载页面
 
更多相关论文
黄秋葵(Abelmoschus esculentus L
基于染料嵌层超分子囊泡的新型二氧
GL公司智力资本管理与绩效激励的融
防范上市公司财务舞弊
契约型证券投资基金所得税纳税主体
全面预算管理在保险业的应用研究
预测概率银行体系的效率:稳定的作
“营改增”试点中存在问题和改进建
税收规避、高管薪酬与企业价值
中间品的生产网络分析
“斑鸠青年商学院社区APP”创业计划
D服务公司技术支持部客户服务水平的
H公司绩效管理的改进
营运资本管理与盈利能力的相关性分
国有建筑企业的企业文化建设困境及
“美禄”儿童乳饮品营销策略研究
X卷烟制造企业核心能力提升研究
卷烟需求预测模型研究
内蒙古胜丰四社的向日葵种植研究
论著作权法中的收回权
基于mRMR结合SVM-forward特征选择的
基于地理位置的社交网络中的区域推
MgZnO准三元混晶的制备及其生长工艺
基于激励理论的厦大饮食中心薪酬制
金砖国家高等学校分类研究
栏 目 导 航
 
 
黄秋葵(Abelmoschus esculentus L.)论文 组织培养论文 体外褐变 抗褐变剂论文 花青素生产论文 次级抗氧化剂
版权申明:目录由用户cqx3**提供,www.51papers.com仅收录目录,作者需要删除这篇论文目录请点击这里
| 设为首页||加入收藏||站内搜索引擎||站点地图||在线购卡|
版权所有 教育论文网 Copyright(C) All Rights Reserved